Cetylpyridinium Chloride Induces Resistance Genes in Candida Albicans

Aim: The antimicrobial cetylpyridinium chloride (CPC) is used in the management of denture stomatitis-associated oral candidiasis as an alternative therapy well for hygiene. Cetylpyridinium survives cavity long periods at low doses, which fluctuates due to dynamics cavity. In this study, it was aimed evaluate impact different sub-therapeutic CPC concentrations (1/100, 1/200) time (0.,5., 2., 24., 48. h) on expression drug-resistance genes (CDR1, CDR2, MDR1, ERG11) Candida albicans SC5314. Method: Total RNA extracted immediately after exposure using Biospeedy® Tri-Easy Isolation Kit followed by Real-Time Quantitative Reverse Transcription polymerase chain reaction (qRT-PCR). data were analyzed comparative 2-ΔΔCT method calculate relative target treatment with concentrations, standardized housekeeping gene Actin. Results: found that drug resistance levels increased high (1/100) 48 h, whereas downregulated 1/200. Conclusion: These results may provide insight into mechanisms action and aid development future strategies therapy.